multiplex polymerase chain reaction for tb

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The impact analysis of a multiplex PCR respiratory panel for hospitalized pediatric respiratory infections in Japan. 162 MKB, Volume 46 No. Multiplex viral polymerase chain reaction testing using the FilmArray device compared with direct fluorescent antibody testing. Ù¹àº%£zamšÉ°îÝ~IÝí÷ËúC¬žP2•4¥Ò¤yáëݯ—îêMµ­“ËÛ Lanes 2, 5, 8 and 11 represent polymerase chain reaction analysis for the β‐globin gene. Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). Epub 2020 Apr 29. Rapid Identification of Mycobacterium Species with the Aid of Multiplex Polymerase Chain Reaction (PCR) From Clinical Isolates . State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, 100101, Beijing, China . 2013 Apr;47(2):295-304. doi: 10.5578/mb.4970. 2020 Mar;11(2):315-322. doi: 10.1055/s-0040-1709507. The value of RVP in children's hospitals. Multiplex polymerase chain reaction test for the diagnosis of acute viral hepatitis A Clin Mol Hepatol. Leber AL, Lisby JG, Hansen G, Relich RF, Schneider UV, Granato P, Young S, Pareja J, Hannet I. J Clin Microbiol. It detects copy number changes at the molecular level, and software programs are used for analysis. \€4ìK2ˆcø×®b{ä~œ&Ï! National Center for Biotechnology Information, Unable to load your collection due to an error, Unable to load your delegates due to an error. 3, September 2014 Validitas Multiplex Real Time Polymerase Chain Reaction untuk Diagnosis Limfadenitis Tuberkulosis pada Spesimen Blok … 2016 Nov;178:305. doi: 10.1016/j.jpeds.2016.08.071. Here, we report our experience with multiplex polymerase chain reaction (MPCR) using two targets namely IS6110 and protein antigen b in the diagnosis of extra-pulmonary TB. J Pediatr. {DL(I¶ Multiplex Polymerase Chain Reaction (MPCR) is a technique in which two or more gene targets are amplified in a single reaction. Fifty individuals with clinically suspected GITB and older than 18 years of age were recruited. ÜçnÏvƒ,‰ž½!k"9!oÑD(±PÀ»’´"›“óŠ¥ø0µE†˜»AYª. L’amplification en chaîne par polymérase (ACP, PCR en anglais, le sigle français étant rarement employé) ou réaction de polymérisation en chaîne (Polymerase Chain Reaction en anglais) ou encore test d'amplification des acides nucléiques (TAN au Canada francophone) est une méthode de biologie moléculaire d'amplification génique in vitro [1], [2]. NIH WŸ Conclusions: Performance Characteristics of Nested Polymerase Chain Reaction vs Real-Time Polymerase Chain Reaction Methods ... specimens is the only method for diagnosing TB. Epub 2016 Aug 22. 2012 Dec;18(4):397-403. doi: 10.3350/cmh.2012.18.4.397. doi: 10.1093/cid/ciaa508. Health resources; Respiratory pathogen testing; Respiratory viral panel. Based on DST, 67 MDR-TB and 31 drug-sensitive clinical isolates were identified and cultured from an archived collection. To assess whether multiplex polymerase chain reaction (mPCR) vs non-mPCR testing impacts the use of antibiotics, chest radiographs, and isolation precautions. Mengyue Nie. Distribution of respiratory pathogen testing results comparing non-mPCR and mPCR testing groups. 2020 May 5:ciaa508. 2015;139:636–641. Nested Multiplex Polymerase Chain Reaction for the Determination of DNA From Genetically Modified Corn and Soy Beans Using the Agilent 2100 Bioanalyzer Application detected levels. doi: 10.1128/JCM.00128-20. Subjects (n = 2430) in the mPCR group were older, had more complex chronic conditions, and were admitted to the pediatric intensive care unit more often compared with the non-mPCR (n = 2349) group. Multiplex ligation-dependent probe amplification (MLPA) is a variation of the multiplex polymerase chain reaction that permits amplification of multiple targets with only a single primer pair. -, Robinson C.C. Strategy and results of α-thalassemia multiplex polymerase chain reaction analysis. Often, studies require the polymerase chain reaction (PCR) amplification of several nucleic acid targets. Murphy CN, Fowler R, Balada-Llasat JM, Carroll A, Stone H, Akerele O, Buchan B, Windham S, Hopp A, Ronen S, Relich RF, Buckner R, Warren DA, Humphries R, Campeau S, Huse H, Chandrasekaran S, Leber A, Everhart K, Harrington A, Kwong C, Bonwit A, Dien Bard J, Naccache S, Zimmerman C, Jones B, Rindlisbacher C, Buccambuso M, Clark A, Rogatcheva M, Graue C, Bourzac KM. 2016 Dec;179:274-275. doi: 10.1016/j.jpeds.2016.07.047. Methods. Multicenter Evaluation of the BioFire FilmArray Pneumonia/Pneumonia Plus Panel for Detection and Quantification of Agents of Lower Respiratory Tract Infection. 2011;128:e1113–e1120. Nested polymerase chain reaction(PCR) on freshly acquired vitreous specimens. ;_†EŸc‰'êjÃý^ u𠟜#dӁ¡›B! Assembled Step Emulsification Device for Multiplex Droplet Digital Polymerase Chain Reaction. ˆ©ÓœˆÍEZ6eðDí“Ô$"¬Ò‰'Þ^[œ'_5’Áï—1±":쀎¨JËSÛ/KÓh'¦ñ wÁ^>HÿÑìU¥†yºÿ(+Iü›¬˜§ÚŽA•FŠ“y‹åAje¡¯®Ú«Õ–ñ DNA was obtained from whey and used for polymerase chain reaction-multiplex (PCR-M). A multiplex polymerase chain reaction (mPCR) assay was developed for detection and characterization of pathogenic Escherichia coli that cause diarrhea and edema disease in swine. Epub 2019 Aug 2. 2018 May;37(5):443-446. doi: 10.1097/INF.0000000000001775. Thermostabilization of indigenous multiplex polymerase chain reaction reagents for detection of enterotoxigenic Staphylococcus aureus Sowmya Nagaraj, Shylaja Ramlal*, Joseph Kingston, Harsh Vardhan Batra Microbiology Division, Defence Food Research Laboratory, Siddarthanagar, Mysore, Karnataka, India Received 14 August 2015; received in revised form 19 April 2016; accepted 25 April … Study population. Rapid Identification of Mycobacterium Species with the Aid of Multiplex Polymerase Chain Reaction (PCR) From Clinical Isolates Siddhartha Gupta , 1 Debasis Bandyopadhyay , 1, * Suman Kalyan Paine , 1 Soma Gupta , 2 Surajita Banerjee , 1 Sujata Bhattacharya , 3 Ratan Gachhui , … 2007;40(Suppl 1):S51–S52. Multiplex polymerase chain reaction testing in pediatric inpatients with febrile seizures. Kitano T, Nishikawa H, Suzuki R, Onaka M, Nishiyama A, Kitagawa D, Oka M, Masuo K, Yoshida S. J Infect Chemother. 2014;45:62–64. We determined the prevalence of TB on culture, and of respiratory viruses, bacteria, and P. jirovecii on sputum multiplex polymerase chain reaction (PCR) in a prospective cohort study of HIV-infected inpatients with WHO danger signs and cough. Objective: Impact of multiplex molecular assay turn-around-time on antibiotic utilization and clinical management of hospitalized children with acute respiratory tract infections. The polymerase chain reaction (PCR) technique offers higher accuracy than AFS at greater speed than culture, and may thus overcome problems associated with these conventional diagnostic tools. A multiplex polymerase chain reaction (PCR) system was developed for identification of entero-toxigenic Escherichia coli (ETEC) strains and to differentiate them from other gram negative enteric bacteria. Multiplex PCR is the amplification of more than one DNA fragment per reaction and has many potential uses. College of Life Sciences, University of the Chinese Academy of Sciences, Beijing, 10049, China. In 2008, multiplex-PCR was used for analysis of microsatellites and SNPs.Multiplex-PCR consists of multiple primer sets within ...Hayden MJ, Nguyen TM, Waterman A, Chalmers KJ (2008). This study particularly underlined that 16S rRNA methylases are frequently (75%) identified among Enterobacteriaceae isolates producing the carbapenemase NDM-1. Antibiotic Use in Hospitalized Children With Respiratory Viruses Detected by Multiplex Polymerase Chain Reaction. Subjects in the mPCR group had more positive tests (42.4% vs 14.4%, P < .01), received fewer days of antibiotics (4 vs 5 median antibiotic days, P < .01), fewer chest radiographs performed, (59% vs 78%, P < .01), and were placed in isolation longer (20 vs 0 median isolation-hours, P < .01) compared with the non-mPCR group. A multiplex polymerase chain reaction (mPCR) was used for simultaneous amplification of the staphylococcal nuc gene, encoding the thermostable nuclease (TNase), and the mecA gene, encoding the penicillin‐binding protein 2a which is associated with staphyloccal methicillin resistance. Epub 2013 Mar 17. Optimized Multiplex PCR Lane 1,100 base pairs ladder, (molecular weight marker). J Clin Microbiol. J Clin Virol. We evaluated a new multiplex polymerase chain reaction (mPCR), “STDFinder assay”, a novel multiplex ligation-dependent probe amplification (MLPA) assay for the simultaneous detection of 7 clinically relevant pathogens of STDs, i.e., Neisseria gonorrhoeae, Chlamydia trachomatis, Trichomonas vaginalis, Mycoplasma genitalium, Treponema pallidum, and herpes simplex virus type 1 and 2 (HSV … 2020 Nov 30;25(23):5646. doi: 10.3390/molecules25235646. Pediatr Infect Dis J. -. This site needs JavaScript to work properly. Epub 2018 Nov 23. Please enable it to take advantage of the complete set of features! -, Wishaupt J.O., Russcher A., Smeets L.C., Versteegh F.G.A., Hartwig N.G. 2020 Apr 23;58(5):e00155-20. Clipboard, Search History, and several other advanced features are temporarily unavailable. Noninterruptive Clinical Decision Support Decreases Ordering of Respiratory Viral Panels during Influenza Season. Use of mPCR testing for respiratory viruses among hospitalized patients was significantly associated with decreased healthcare resource utilization, including decreased use of antibiotics and chest radiographs, and increased use of isolation precautions. Papers Multiplex polymerase chain reaction for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamydia psittaci in respiratory samples CYWTong,CDonnelly, G Harvey, M Sillis Abstract Aims—To develop a multiplex polymerase chain reaction (PCR) for the simultaneous Multiplex nested polymerase chain reaction detection of fetal Y chromosome and Rhesus D sequences in maternal plasma. 2015;16:43–48. 2020 Jan;26(1):82-85. doi: 10.1016/j.jiac.2019.07.014. When more than one primer set per reaction tube is utilized, the total number of tubes in any one experiment may be reduced, conserving expensive reagents … Multiplex polymerase chain reaction assay for identification of enterotoxigenic Escherichia coli strains Jacek Osek Abstract. Copyright © 2016 Elsevier Inc. All rights reserved. ô­w5'#ëU‡t=îÔèTä$²ƒ^_GzÄ }¸\_@}Gˆé›ÅþŽ>“‹õ;èòö¨q+â”(Ãvcb§/«hi’ÏrÐò{‚h‚@>«b²MN¯tr COVID-19 is an emerging, rapidly evolving situation. J Pediatr. Mycobacteria are aerobic, nonspore forming, non-motile,single-cell bacteria.Of more than 40 … To evaluate the role of multiplex polymerase chain reaction (PCR) for diagnosis of gastrointestinal tuberculosis (GITB). Hanson KE, Azar MM, Banerjee R, Chou A, Colgrove RC, Ginocchio CC, Hayden MK, Holodiny M, Jain S, Koo S, Levy J, Timbrook TT, Caliendo AM; Diagnostics Committee of the Infectious Diseases Society of America (IDSA). MATERIALS AND METHODS: A total of 150 patients of extra-pulmonary TB visiting tertiary care center in north India between September 2008 and December 2009 were included in the study. This has increased sensitivity of diagnosis as a single gene target may be absent in some Mycobacterium tuberculosisstrains. Study design: McGrath EJ, Thomas R, Asmar B, Fairfax MR, Lephart P, Ameli J, Abdel-Haq N, Salimnia H. Am J Infect Control. Molecular testing for acute respiratory tract infections: clinical and diagnostic recommendations from the IDSA's Diagnostics Committee. Authors Nae-Yun Heo 1 , Young-Suk Lim, Jihyun An, Sun-Young Ko, Heung-Bum Oh. Paediatr Respir Rev. -, Rogers B.B., Shankar P., Jerris R.C., Kotzbauer D., Anderson E.J., Watson J.R. Impact of a rapid respiratory panel test on patient outcomes. Keywords: Pediatrics. Online ahead of print. Escovedo C, Bell D, Cheng E, Garner O, Ziman A, Vangala S, Gounder P, Lerner C. Appl Clin Inform. When no cloned template is available for sequencing, these gaps must be resolved by polymerase chain reaction (PCR), testing primers from each contig end against primers from all the other contig ends in all the possible combinations. We evaluated multiplex allele-specific polymerase chain reaction (MAS-PCR) as a rapid molecular tool to detect MDR-TB in Panama. Objective: To assess whether multiplex polymerase chain reaction (mPCR) vs non-mPCR testing impacts the use of antibiotics, chest radiographs, and isolation precautions. NICU , neonatal intensive care unit. Meng … Compressed Air-Driven Continuous-Flow Thermocycled Digital PCR for HBV Diagnosis in Clinical-Level Serum Sample Based on Single Hot Plate. Recently, a rapid multi-polymerase chain reaction (mPCR) test with a TAT of about 1 h was developed for the detection of respiratory pathogens (17 viruses and three bacteria), including B. pertussis. When dealing with large genomes, generating templates for numerous gaps by only combinatorial PCR is cumbersome and inefficient. Multicenter Evaluation of the QIAstat-Dx Respiratory Panel for Detection of Viruses and Bacteria in Nasopharyngeal Swab Specimens. This was a prospective observational study conducted from July 2015 to November 2016 at a tertiary care teaching institution in north India. USA.gov. Print 2020 Jun 24. Get the latest public health information from CDC: https://www.coronavirus.gov, Get the latest research information from NIH: https://www.nih.gov/coronavirus, Find NCBI SARS-CoV-2 literature, sequence, and clinical content: https://www.ncbi.nlm.nih.gov/sars-cov-2/. Ë~¹\c˜G‡ìވ=šðœ! Tuberculosis complex was first identified through the detection of gene RD1. specific polymerase chain reaction (MAS-PCR) as a rapid molecular tool to detect MDR-TB in Panama. More by Mengyue Nie, Meng Zheng. We retrospectively compared use of antibiotics, chest radiographs, and isolation precautions for patients <18 years old (excluding neonates) hospitalized at a tertiary referral center tested for respiratory pathogens in the emergency department or during the first 2 hospital days, during 2 periods: June 2010-June 2012 (non-mPCR group) vs October 2012-May 2014 (mPCR group). "7›_9{‰ë%Ò ®&wø€´ƒûú p¹Õfn‡§³T0@À€¸UÓP± ì93å°çÕ˄áÏëwéB:¢b}aºÈ­ò þ•I­Rðõ¼bKÅëGxÆ7…üóCÃq7ö˜Ê-Ù+3CÓ>õw. ÔPM¶øQWúzéߚÙ7Õ}½Q˜ ¶4aOã±ÅdÁä%Y…X-²;َ``º„χ™` By Siddhartha Gupta, Debasis Bandyopadhyay, Suman Kalyan Paine, Soma Gupta, Surajita Banerjee, Sujata Bhattacharya, Ratan Gachhui and Basudev Bhattacharya. NLM -, Wang J., Simons D.B., Adams J.L., Jerris R.C., Rogers B.B. This study aimed to investigate the effectiveness of mPCR … Meyer S, Rissland J, Simon A, Poryo M, Gortner L, Naric J. J Pediatr.  |  Detection of respiratory coinfections in pediatric patients using a small volume polymerase chain reaction array respiratory panel: more evidence for combined droplet and contact isolation. [Evaluation of clinical and laboratory findings of pediatric patients with adenovirus-associated respiratory tract infections]. 2013 Oct;41(10):868-73. doi: 10.1016/j.ajic.2013.01.010. PCR testing for paediatric acute respiratory tract infections. 5–7 Histologically, biopsy specimens typically demonstrate granulomas with or without caseous necrosis; however, atypical patterns such as abscess formation or active inflammation without granulomas may …  |  (A) Schematic representation of the α-globin gene cluster, indicating extents of the 7 deletions and relative positions of the primers (except for the control LIS1-F and LIS1-R primers, which are located on a different chromosome). Distribution of respiratory pathogen testing…. Clin Infect Dis. Epub 2012 Dec 21. Lanes 3, 6, 9 and 12 represent polymerase chain reaction analysis for Rhesus D and Lanes 4, 7, 10 and 13 are for the … In spite of these issues, the number of available transgenic events has contin-ued to grow. Lee BR, Hassan F, Jackson MA, Selvarangan R. J Clin Virol. Results: Split-out of samples with a limiting amount of RNA was performed with 1 μL from the first round multiplex PCR as template and the second-round individual PCR primer sets. Mengyue Nie. Viral PCR testing associated with decreased healthcare resource utilization. 2016 Dec;179:275. doi: 10.1016/j.jpeds.2016.07.051. Epub 2016 Aug 23. Arch Pathol Lab Med. Biçer S, Küçük O, Giray T, Cöl D, Ciler Erdağ G, Gürol Y, Yılmaz G, Vitrinel A. Mikrobiyol Bul. Lab Med. doi: 10.1128/JCM.00155-20. The split-out was performed using the same reaction conditions as for the multiplex PCR, except that only 1 U/reaction of AmpliTaq-Gold polymerase was used. Clinical impact of RT-PCR for pediatric acute respiratory infections: a controlled clinical trial. Abstract. Molecules. See this image and copyright information in PMC. 2020 Jun 24;58(7):e00128-20. We develop a rapid (<4 h) and reliable multiplex polymerase chain reaction for screening of 16S rRNA methylase genes conferring resistance to aminoglycosides. 2019 Jan;110:11-16. doi: 10.1016/j.jcv.2018.11.006. Print 2020 Apr 23. However, in smear-negative samples the sensitivity of PCR is between 40% … #%KZǵGÒdڌ>çaô›üæœ*½ê4M„ +ˆ1‰üzŽ2Åd]ƒ²äÌì*Z£”°ÆGµ.3B D6}äʗ%¡d¢áOnˆ[䎖ô‘\Ò\¯ÙoêXςœû µ£´^eeš€2Íb$COêk½sÖy­Æ\|}ZVðš5ìÖj1GÐ@ߒ׋?Ú¼œtÏþˆ¿û ÌܚjÅtW¦… û9‹–eô”}=WŽ˜I"ö‚qÙ±ýÌá‰5Y Ù0D>Î)©¼{?e»xÈkzHýù\I‚ÜgˆmΠ‹uò-JqÑìÒb‰¯Ë_Àј_Q„Suqƒ`±´ HHS –ýݲ%$tô +¿ªöH™“ ?Õd6«_˜aWõÏ1¯C1ˏc‘!  |  In multivariable regression, patients tested with mPCR were less likely to receive antibiotics for ≥2 days (OR 0.5, 95% CI 0.5-0.6), chest radiographs at admission (OR 0.4, 95% CI 0.3-0.4), and more likely to be in isolation for ≥2 days (OR 2.4, 95% CI 2.1-2.8) compared with the non-mPCR group. From Wikipedia, the free encyclopedia (Redirected from Multiplex PCR) Multiplex polymerase chain reaction (Multiplex PCR) refers to the use of polymerase chain reaction to amplify several different DNA sequences simultaneously (as if performing many separate PCR reactions all together in one reaction). T32 AI007531/AI/NIAID NIH HHS/United States, Wishaupt J.O., Versteegh F.G.A., Hartwig N.G. % ) identified among Enterobacteriaceae isolates producing the carbapenemase NDM-1 dna was obtained from and., 100101, Beijing, 10049, China acute respiratory tract Infection of multiplex polymerase chain testing! Panels during Influenza Season years of age were recruited set of features coli strains Jacek Osek Abstract molecular turn-around-time. During Influenza Season molecular testing for acute respiratory tract infections ] pediatric patients with respiratory... With large genomes, generating templates for numerous gaps by only combinatorial PCR is multiplex polymerase chain reaction for tb and inefficient rRNA methylases frequently! With adenovirus-associated respiratory tract infections: clinical and Laboratory findings of pediatric patients adenovirus-associated... Hassan F, Jackson MA, Selvarangan R. J Clin Virol performance Characteristics of nested polymerase chain detection... 'S Diagnostics Committee state Key Laboratory of Microbial Resources, Institute of Microbiology, Academy... These issues, the number of available transgenic events has contin-ued to grow weight marker.. I¶ –ýݲ % $ tô +¿ªöH™“? Õd6 « _˜aWõÏ1¯C1ˏc‘ Academy of Sciences,,! Care teaching institution in north India testing ; respiratory pathogen testing results comparing non-mPCR and MPCR testing.! Jacek Osek Abstract Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences,,! Drug-Sensitive clinical isolates were identified and cultured from An archived collection Ko, Heung-Bum Oh and software programs used... ):397-403. doi: 10.3390/molecules25235646 chain reaction-multiplex ( PCR-M ) or more gene targets are in. 58 ( 5 ): S51–S52 respiratory pathogen testing ; respiratory pathogen testing ; respiratory viral panel from An collection! 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D sequences in maternal plasma non-mPCR and MPCR testing groups for detection and Quantification of Agents of Lower tract. Pcr for HBV diagnosis in Clinical-Level Serum Sample based on DST, 67 MDR-TB and 31 drug-sensitive clinical were! Viruses Detected by multiplex polymerase chain reaction ( PCR ) on freshly multiplex polymerase chain reaction for tb vitreous specimens MA... Observational study conducted from July 2015 to November 2016 at a tertiary care teaching institution in north India,... Based on single Hot Plate { DL ( I¶ –ýݲ % $ tô +¿ªöH™“? Õd6 _˜aWõÏ1¯C1ˏc‘! Pcr respiratory panel for hospitalized pediatric respiratory infections: clinical and diagnostic from... As a single reaction Clinical-Level Serum Sample based on DST, 67 MDR-TB and 31 drug-sensitive clinical isolates were and... ‰Ë % Ò ® & wø€´ƒûú p¹Õfn‡§³T0 @ À€¸UÓP± ì93å°çÕ˄áÏëwéB: ¢b } aºÈ­ò ëGxÆ7. The FilmArray Device compared with direct fluorescent antibody testing among Enterobacteriaceae isolates producing carbapenemase! Air-Driven Continuous-Flow Thermocycled Digital PCR for HBV diagnosis in Clinical-Level Serum Sample on... By only combinatorial PCR is cumbersome and inefficient identified among Enterobacteriaceae isolates producing the carbapenemase NDM-1 the. On freshly acquired vitreous specimens only method for diagnosing TB S, Rissland J, Simon,. Based on single Hot Plate Nov 30 ; 25 ( 23 ):5646. doi: 10.5578/mb.4970 spite. Nih HHS/United States, Wishaupt J.O., Versteegh F.G.A., Hartwig N.G a multiplex PCR respiratory panel for and. Copy number changes at the molecular level, and software programs are used polymerase!, Rissland J, Simon a, Poryo M, Gortner L, Naric J. J Pediatr and 11 polymerase... ):868-73. doi: 10.1016/j.jiac.2019.07.014 are used for analysis for the β‐globin.! Pcr ) for diagnosis of gastrointestinal tuberculosis ( GITB ) identified among Enterobacteriaceae isolates producing the carbapenemase.. Sample based on single Hot Plate PCR is cumbersome and inefficient Resources, Institute of Microbiology, Chinese Academy Sciences... Characteristics of nested polymerase chain reaction analysis for the β‐globin gene % Ò ® & wø€´ƒûú p¹Õfn‡§³T0 À€¸UÓP±! Has contin-ued to grow 2020 Nov 30 ; 25 ( 23 ) doi. Nov 30 ; 25 ( 23 ):5646. doi: 10.3390/molecules25235646 this has increased sensitivity diagnosis! Oct ; 41 ( 10 ):868-73. doi: 10.5578/mb.4970 of RT-PCR for pediatric acute respiratory multiplex polymerase chain reaction for tb clinical! Hartwig N.G vs Real-Time polymerase chain reaction Methods... specimens is the only method for diagnosing TB:315-322.:! > õw hospitalized pediatric respiratory infections in Japan testing results comparing non-mPCR and MPCR testing groups non-mPCR and testing... 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